Journal: 3d Printing and Additive Manufacturing

Article Title: Three-Dimensional Printed Stamps for the Fabrication of Patterned Microwells and High-Throughput Production of Homogeneous Cell Spheroids

doi: 10.1089/3dp.2019.0129

Figure Lengend Snippet: Agarose microwell fabrication process. (A) Sterilization of the stamp, (B) insertion of the stamp into a 24-well tissue culture plastic well, (C) pipetting of the melted 1.5% agarose into the well using the stamp channels to guide the agarose pipetting and allow for air displacement, (D) agarose gelation for 5 min at room temperature, and (E) removal of the stamp using the stamp stopper at the top to obtain patterned agarose microwells. Figure created with BioRender.

Article Snippet: Stamp sterilization The stamps were sterilized in ethylene oxide (EtO) using an EtO sterilization chamber (Andersen Sterilizers, Haw River, NC) and a 24 h sterilization cycle 48 h before stamp use for the fabrication of the agarose microwells.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Adipose mesenchymal stromal/stem cells expanded by a GMP compatible protocol displayed improved adhesion on cancer cells in flow conditions

doi: 10.21037/atm.2020.04.25

Figure Lengend Snippet: Schematic representation of devices used to perform ASC adhesion and detachment assays. The parallel flow chamber slide (see photographic enlargement), was placed on an inverted epifluorescence microscope. Washed ASC after fluorescent labeling were resuspended with perfusion medium in a conical tube (Reservoir). In controlled environment (37 °C; standard atmospheric pCO2 and humidity) ASC were perfused in the parallel flow chamber through a small bore catheter. Flow was imposed by a syringe pump (Harvard apparatus). Adherent and detaching ASC were visualized by a digital camera and the number of adherent cells was estimated by image analysis approach. Timing of flow rate regulation and of digital image capture was reported in methods section and in Figure 1A. Dashed arrows represent the direction of flowing medium containing labeled ASC. Dashed orange box encloses devices placed under controlled environmental conditions.

Article Snippet: Experiments were performed taking advantage of a sterile disposable parallel flow chamber (ibiTreat - Slide I Luer, Ibidi GmbH, Gräfelfing, Germany).

Techniques: Inverted Epifluorescence, Labeling